Hi Jen, I can really sympathise with you - earlier this year I was at a stage where my experiments just did not work, and I had no help from anyone becuase they were all so busy. I really felt like giving up. I eventually explained my problems to my supervisor, and we sorted it out. There have been many times that I've cried in the bog - so don't feel ashamed about that!! You should eapproach someone about this before it gets worse - don't be scared of your supervisor. Things have worked out so much better for me since I did that.