he helped me to optimise the method and thought it was fine but he seems to think im im doing something wrong in the mastermix but i dont see how!

i have three biological replicates then three technical replicates also. the results i have obtained have been from two different types of samples, but each experiment giving data going against the puplished dataset. superivisor has been less than helpful basically saying it has to be wrong!

Stevie wonder Superstitious, cant beat the old ones.

i suggested this to him but he is unwilling. think i might go over his head and speak with someone else.

true but then i have no reason to think they are incorrect as such when the numbers match the published papers, its just they dont indicate the same things.
ill just have to come up with a good defence for my viva then!

when i say first set i mean from the data that will make up my thesis as in also have done several other experiments along a similar line and found the same thing, but as running out of time these are the first set that will go into thesis.. if that makes sense! in terms of literature there is only one group who has done this and a handful of papers.. am i so naive to think that their results may not be conclusive?

well today i achieved my first set of results. and they look pretty good with great error bars and replicates pretty good. except they dont agree with the few papers in the literature, so my sup decided i did the experiment wrong. except i have no reason to doubt the experiment didnt work. The controls were exactly the same as the previous trials and nothing in the protocol was changed. how do i say i think ive done it right its just that it doesnt prove his theory thats the problem (this has been a running theme in my project where if its not as he expects he says ive done it wrong)

apparently if there are locks on doors it changes the letting terms and is no longer let a 'house' so the rules are different for the owner. also it means each room needs a tv licence.

i had mixed experiences with teachers and PhDs, one teacher who got one whilst i was there seemed to adopt a bit of an attitude and felt teaching was now below her. another didnt really know how to teach at our level and literally tried to teach us her uni biology which was way advanced of A level. I dont think a Phd can make you a good teacher, training can.

they look like gardeners shoes...!

i feel for you i really do i gues if she knew it was over she would have said, and if you can find a way of communicating to her that you feel there is a future it might help her feel more secure.

i undertsand how hard that is waiting for 'that call' that lets you know where you stand.

hope things work out for you. sounds like your taking the best route letting her know your there but not crowding her.

ah ok i see. well maybe she will suggest un paid placement type job that would help you gain the experience that you would need though i would imagine would involve a generous amount of time to gain relevant skills.
maybe she is impressed by your keen nature and it may even lead on to a PhD afterwards.

if the experience is whats stopping you getting a PhD maybe a year spend being a RA or hospital lab assistant etc is the way to go, to also make sure you know it is what you want to do

im bit confused do you mean she offered you one but your turned it down because you didnt have experience?