Hi,
I was wondering if anyone had any experience in the use of mounting media to attach coverslips to tissue sections once they have been stained? These sections have been processed into paraffin wax and so are de-waxed and re-hydrated prior to staining (it's an aqueous stain)
I have been using DPX but this renders the sample optically transparent. Whilst this is great for light transmittance it hinders the ability to be able to see the original structures prior to applying the DPX.
If anyone knows of any tried and tested alternative permanent mounting medium to DPX that might alleviate these problems, then your help would be much appreciated.
Sorry for the off-topic post...
Thanks!!