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quick ligation question...

K

I've posted this on protocol online too but need an answer asap so thought I'd try here too. When doing ligations I usually incubate at 16 C overnight. But was wondering if there is a maximum time I can leave it? Could I set it up now (11:30am) and then do the transformation tomorrow at 9am, more than 20 hours incubation? Thank you to anyone who can help

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