In my recent reference, my supervisor highlighted the fact that I work very promptly, have great publications coming out, am very organised and juggle multiple projects, good at teaching (I am amazing obviously at everything).
If you're going to just have 1 reference, then make sure its a balance of teaching/research mention publications and any other strengths etc. because some jobs will be full on teaching, some will be purely research, some will be a mix, some will require you to have worked with xyz participants etc. so a balanced reference should serve you well.