Hi,
Thanks for the advice - turned out alright in the end! the only issue is some SEM - I have an n of 5 western blots, but two conditions on one western blot aren't applicable (basically I can't measure the ratio between two of my proteins as one isnt there). It doesnt change the average that much but these two are in one sense an n of 4, but in another an n of 5 so not sure which way to calculate the SEM (so i calculate it as Std deviatin/square root of repeats)