thanks to all of you who have replied me , encouraged me and helped me to sort it out. Yes, I am sticking to it..and will be hopefully...

thanks all for helping me out.. but I am still there, I peformed the radiolabelled binding assay last week again. still its not giving good scathcard curve.. last night i was thinking of quiting...I am so p**sed of..

I am really scared. I have been trying to perform an assay for last 5 monhts.. But the data are not reproducible at all. I am very much scared. The supervisor also got angry on me...what will I do? I have modified lots of factors, considered several things..now I am really frustrated. feeling nowhere..is it a common feeling. BTW I have just finished my first year.