How can you visualise BrdU (bromodeoxyuridine)-positive nuclei by using DAB (diaminobenzidine) ?
I know that you can do this by adding a primary Ab against BrdU, then a secondary Ab congugated with horseadish peroxide (HRP), and then adding DAB with hydrogen peroxide, so that in the presence of the HRP the DAB will stain brown.
Is there a way of doing it without using HRP?

I am trying to understand a study and the only thing I'm stuck on is that part. The reason is that they do not say anything about HRP, though they do say about adding primary and secondary Abs and DAB with H2O2.

Should I assume that they used HRP or could they have used a different method?

Please help me. Thanks.

Hi Seriousperson

Looks to me like you have 2 possible options

Use ECL as a substrate for HRP, commonly used for detection in western blotting applications, for example

Or, you could go for a different secondary, say alkaline phosphatases, a variet of detection substrates are available using this system, so you could try this, just google "alkaline phosphate substrates" and see what you get

Hope this helps you out, cakeman