I don't know specifically it varies from person to person.
Why are you interested in this course? include some background knowledge,but don't try to pretend you know it all
Things related to a project. If you applied for a project you will want to mention other research and how they did it
Where do you see yourself in three years time the best way to answer it is honestly... unless you're doing a PhD because you don't know what you want to do.
Just be honest about the where do you see yourself in Five years. As long as it makes sense, then just tell them... You want a research career in Academia, or you want to be working for big pharma or what? Try to match your five year plan to what the place is good in...
I dunno about the more you prepare. You kinda get to the point where you don't seem natural in your interview and as well as wanting to hear your ideas, they also want to hear about you and get an idea of the sort of person they may be taking on. If you think about it too much you just end up sounding stiff and dull.
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