Hi all
You know when your project is pootling along well and interesting things happening - do you get all excited?
Its not very often but when I have times like that I get really happy and realise I love doing what I do and remember why I started on this journey.
Am I just a wierdo / saddo? We all tend to share on here when things aren't very good but I just wanted to say today that I think we are all quite lucky.
:-)
Good for you, pea.
I know that feeling, I love that feeling. When I get colonies on my transformation plates, bands on my PCR gel, lots of ACGT's in my sequencing...all wonderful, beautiful things that make your heart glad you started your PhD. If I could sing, I would. Hugs and kisses to the world!!!!
Enjoy your day, pea!
katq, I am no PCR expert (far from it). But if I have a PCR that doesn't work the first time, I always try temperature, Mg etc gradients before I try different primers. If you have taken this into account already, ignore my advice and try other primers. Good luck, and have a beer for me when you get product!
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