MCL1 western blots - beta actin normalisation on PostgraduateForum.com

MCL1 western blots - beta actin normalisation

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bilbo012 19 posts
9 years ago

Hi all,

I've been doing western blots for McL1 expression (which is 40kDa), stripping the membrane then reprobing for beta actin (which is 42 kda) as the proteins are quite close together I'm worried that my beta actin isn't beta actin but actually mcl1

However my mcl1 has always been faint or quite dirty, however beta actin is quite clean and strong - should I be worried?

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MCL1 western blots - beta actin normalisation

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