Protein isolation advice

Z

Hi, can anyone recommend a good kit for the extraction of protein from whole insects??

Thanks xxx

A

Are you using the baculovirus system from invitrogen?? Is your protein of interest in a construct and tagged? I've expressed my proteins in SF9 cells and purified them from that ...

A

Sorry- it's late and I'm tired. You were talking of extraction from whole insects .... not just insect cell cultures. Gotta go home soon.

D

hehehe ... good old fashioned methods as demonstrated by insect lovers everywhere! ;)

Z

I was trying to be serious.

3487