I've posted this on protocol online too but need an answer asap so thought I'd try here too. When doing ligations I usually incubate at 16 C overnight. But was wondering if there is a maximum time I can leave it? Could I set it up now (11:30am) and then do the transformation tomorrow at 9am, more than 20 hours incubation? Thank you to anyone who can help
PostgraduateForum Is a trading name of FindAUniversity Ltd
FindAUniversity Ltd, 77 Sidney St, Sheffield, S1 4RG, UK. Tel +44 (0) 114 268 4940 Fax: +44 (0) 114 268 5766
An active and supportive community.
Support and advice from your peers.
Your postgraduate questions answered.
Use your experience to help others.
Enter your email address below to get started with your forum account
Enter your username below to login to your account
An email has been sent to your email account along with instructions on how to reset your password. If you do not recieve your email, or have any futher problems accessing your account, then please contact our customer support.
or continue as guest
To ensure all features on our website work properly, your computer, tablet or mobile needs to accept cookies. Our cookies don’t store your personal information, but provide us with anonymous information about use of the website and help us recognise you so we can offer you services more relevant to you. For more information please read our privacy policy
Agree Agree