Signup date: 27 Jan 2012 at 1:09pm
Last login: 27 Jan 2012 at 1:09pm
Post count: 2
Hi there,
I was wondering if anyone can tell me how to quantify fluorescence
intensity with image J. I don't need to analyze single cells, I just want to measure the fluorescent intensity of an image (or selected area of an image) and compare with others taken under same conditions.
According to the protocol I know that I have to do the following:
1. Convert the color picture to 8it-b black and white scale.
2. Subtract the background fluorescence equivalently for all images by setting the threshold to 50% maximum intensity.
3. To quantify the mean fluorescence
I will appreciate if someone can post a tutorial with screen-shots regarding my question.
Thank you in advance for your help
PostgraduateForum Is a trading name of FindAUniversity Ltd
FindAUniversity Ltd, 77 Sidney St, Sheffield, S1 4RG, UK. Tel +44 (0) 114 268 4940 Fax: +44 (0) 114 268 5766
An active and supportive community.
Support and advice from your peers.
Your postgraduate questions answered.
Use your experience to help others.
Enter your email address below to get started with your forum account
Enter your username below to login to your account
An email has been sent to your email account along with instructions on how to reset your password. If you do not recieve your email, or have any futher problems accessing your account, then please contact our customer support.
or continue as guest
To ensure all features on our website work properly, your computer, tablet or mobile needs to accept cookies. Our cookies don’t store your personal information, but provide us with anonymous information about use of the website and help us recognise you so we can offer you services more relevant to you. For more information please read our privacy policy
Agree Agree